Application
CYP3A4 Antibody (Cat. #AM2065a) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the CYP3A4 antibody detected the CYP3A4 protein (arrow).| Product Name | CYP3A4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYP3A4; CYP3A3; Cytochrome P450 3A4; Albendazole monooxygenase; Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase; Taurochenodeoxy |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 501CT16.1.1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYP3A3 |
|---|---|
| Alternative Names | CYP3A4 CYP3A3 Cytochrome P450 3A4 Albendazole monooxygenase Albendazole sulfoxidase CYPIIIA3 CYPIIIA4 Cytochrome P450 3A3 Cytochrome P450 HLp Cytochrome P450 NF-25 Cytochrome P450-PCN1 Nifedipine oxidase Quinine 3-monooxygenase Taurochenodeoxy |
| Molecular Weight(MW) | 57343 Da |
| Function | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide |
| Tissue Specificity | Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056) According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein |
| Entrez Gene | 1576 |
|---|---|
| Nucleotide Accession | NP_059488.2 |
Application
CYP3A4 Antibody (Cat. #AM2065a) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the CYP3A4 antibody detected the CYP3A4 protein (arrow).| Application Notes | WB:1:100~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS. |
