Application
Western blot analysis of DCTN2 Antibody (N-term) in A2058, A375 cell line lysates (35ug/lane). DCTN2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skin with DCTN2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of DCTN2 (arrow) using rabbit polyclonal DCTN2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DCTN2 gene.Application
DCTN2 Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DCTN2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCTN2; DCTN50; Dynactin subunit 2; 50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; p50 dynamitin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DCTN50 |
|---|---|
| Alternative Names | DCTN2 DCTN50 Dynactin subunit 2 50 kDa dynein-associated polypeptide Dynactin complex 50 kDa subunit p50 dynamitin |
| Molecular Weight(MW) | 44231 Da |
| Function | Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development |
| Tissue Specificity | This DCTN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57~86 amino acids from the N-terminal region of human DCTN2. |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Membrane; Peripheral membrane protein |
| Entrez Gene | 10540 |
|---|
Application
Western blot analysis of DCTN2 Antibody (N-term) in A2058, A375 cell line lysates (35ug/lane). DCTN2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skin with DCTN2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of DCTN2 (arrow) using rabbit polyclonal DCTN2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DCTN2 gene.
Application
DCTN2 Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
