Application
Western blot analysis of DFFB Antibody (N-term) in 293 cell line lysates (35ug/lane). DFFB (arrow) was detected using the purified Pab.Application
DFFB Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DFFB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DFFB Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DFFB Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DFFB; CAD; DFF2; DFF40; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; Caspase-activated nuclease; DNA fragmentation factor 40 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAD DFF2 DFF40 |
|---|---|
| Alternative Names | DFFB CAD DFF2 DFF40 DNA fragmentation factor subunit beta Caspase-activated deoxyribonuclease Caspase-activated nuclease DNA fragmentation factor 40 kDa subunit |
| Molecular Weight(MW) | 39110 Da |
| Function | Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology |
| Tissue Specificity | This DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human DFFB. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 1677 |
|---|
Application
Western blot analysis of DFFB Antibody (N-term) in 293 cell line lysates (35ug/lane). DFFB (arrow) was detected using the purified Pab.
Application
DFFB Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DFFB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DFFB Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
