Application
RAT DLG4 Monoclonal Antibody (C-term) (Cat. #AM1833a) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the DLG4 antibody detected the DLG4 protein (arrow).(8ug/ml)Application
RAT DLG4 Monoclonal Antibody (C-term) (Cat. #AM1833a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RAT DLG4 Monoclonal Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | DGL4 Antibody (C-term) (Rat) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dlg4; Dlgh4; Psd95; Disks large homolog 4; Postsynaptic density protein 95; Synapse-associated protein 90 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 127CT15.8.20 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Dlgh4 Psd95 |
|---|---|
| Alternative Names | Dlg4 Dlgh4 Psd95 Disks large homolog 4 Postsynaptic density protein 95 Synapse-associated protein 90 |
| Molecular Weight(MW) | 80465 Da |
| Function | Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B |
| Tissue Specificity | Detected in brain (at protein level). Brain. Highest levels of isoform 2 in cerebellum, cortex, hippocampus, and corpus striatum |
| Cellular Localization | Cell membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Cell junction, synapse, synaptosome. Note=High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells |
| Entrez Gene | 29495 |
|---|---|
| Nucleotide Accession | NP_062567.1 |
Application
RAT DLG4 Monoclonal Antibody (C-term) (Cat. #AM1833a) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the DLG4 antibody detected the DLG4 protein (arrow).(8ug/ml)
Application
RAT DLG4 Monoclonal Antibody (C-term) (Cat. #AM1833a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RAT DLG4 Monoclonal Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
