Application
Western blot analysis of Dicer1 in MCF7 cell line lysates (35ug/lane). DICER1 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of Dicer1 Antibody (Center)(Cat#AP1901c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Dicer1 Antibody (Center) (AP1901c)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Dicer1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Dicer1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DICER1; DICER; HERNA; KIAA0928; Endoribonuclease Dicer; Helicase with RNase motif |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| WB:1:500~2000 IF:1:100~1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DICER HERNA KIAA0928 |
|---|---|
| Alternative Names | DICER1 DICER HERNA KIAA0928 Endoribonuclease Dicer Helicase with RNase motif |
| Molecular Weight(MW) | 218682 Da |
| Function | Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre- miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2 EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs |
| Tissue Specificity | This Dicer1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 886~916 amino acids from the central region of human Dicer1. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 23405 |
|---|
Application
Western blot analysis of Dicer1 in MCF7 cell line lysates (35ug/lane). DICER1 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of Dicer1 Antibody (Center)(Cat#AP1901c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Dicer1 Antibody (Center) (AP1901c)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Dicer1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:500~2000 IF:1:100~1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
