Application
Western blot analysis of EDNRA antibody (Center) (Cat.# AP6507c) in CEM cell line lysates (35ug/lane). EDNRA (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of EDNRA Antibody (Center)(Cat#AP6507c) with HepG2 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of CEM cells using EDNRA Antibody (Center) (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EDNRA Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EDNRA; ETA; ETRA; Endothelin-1 receptor; Endothelin A receptor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:500~2000 IF:1:50~100 FC:1:200~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ETA ETRA |
|---|---|
| Alternative Names | EDNRA ETA ETRA Endothelin-1 receptor Endothelin A receptor |
| Molecular Weight(MW) | 48722 Da |
| Function | Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3 |
| Tissue Specificity | Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| Entrez Gene | 1909 |
|---|
Application
Western blot analysis of EDNRA antibody (Center) (Cat.# AP6507c) in CEM cell line lysates (35ug/lane). EDNRA (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of EDNRA Antibody (Center)(Cat#AP6507c) with HepG2 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of CEM cells using EDNRA Antibody (Center) (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:500~2000 IF:1:50~100 FC:1:200~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
