WB
Western blot analysis of extracts from COS-7 cells, using EDNRA antibody.WB
Western blot analysis of extracts from Hela, using EDNRA Antibody. Lane 1 was treated with the blocking peptide.IHC
EDNRA Antibody at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.IHC
EDNRA Antibody at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Product Name | EDNRA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human EDNRA, corresponding to a region within C-terminal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EDNRA |
|---|---|
| Gene Synonyms | ETA ET-A ETAR ETRA MFDA ETA-R hET-AR |
| Gene Full Name | endothelin receptor type A |
| Gene Summary | This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| Alternative Names | Ednra EDNRA_HUMAN Endothelin 1 receptor precursor Endothelin 1 specific receptor Endothelin A receptor Endothelin ET A receptor Endothelin receptor ET1 specific type Endothelin receptor subtype A Endothelin receptor type A Endothelin-1 receptor ET A ET-A ETA ETA R ETA-R ETAR ETRA G protein coupled receptor hET AR hET-AR MFDA |
| Molecular Weight(MW) | 48 KD; 49kD(Calculated). |
| Cellular Localization | Cell membrane>Multi-pass membrane protein. |
WB
Western blot analysis of extracts from COS-7 cells, using EDNRA antibody.
WB
Western blot analysis of extracts from Hela, using EDNRA Antibody. Lane 1 was treated with the blocking peptide.
IHC
EDNRA Antibody at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
IHC
EDNRA Antibody at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
