Application
Flow cytometric analysis of CEM cells using EDNRA Antibody (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of EDNRA Antibody with HepG2 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of EDNRA antibody in CEM cell line lysates (35ug/lane)| Product Name | EDNRA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Endothelin-1 receptor, Endothelin A receptor, ET-A, ETA-R, hET-AR, EDNRA, ETA, ETRA |
| Product description | EDNRA is a receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.1) Cardillo,C., Arthritis Rheum. 60 (6), 1840-1844 (2009) Hayzer,D.J., Am. J. Med. Sci. 304 (4), 231-238 (1992) |
| Immunogen | This EDNRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human EDNRA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | EDNRA |
|---|---|
| Alternative Names | Endothelin-1 receptor Endothelin A receptor ET-A ETA-R hET-AR EDNRA ETA ETRA |
| Molecular Weight(MW) | 49 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of CEM cells using EDNRA Antibody (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of EDNRA Antibody with HepG2 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of EDNRA antibody in CEM cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
