Application
Western blot analysis of EEF1A1 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of EEF1A1 Antibody (C-term)(Cat#AP6592b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
EEF1A1 Antibody (C-term) (AP6592b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EEF1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | EEF1A1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIFIHC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EEF1A, EF1A, LENG7 |
|---|---|
| Alternative Names | EEF1A1 EEF1A EF1A LENG7 Elongation factor 1-alpha 1 Elongation factor Tu Eukaryotic elongation factor 1 A-1 Leukocyte receptor cluster member 7 |
| Molecular Weight(MW) | 50141 Da |
| Function | This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production |
| Tissue Specificity | Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle |
| Cellular Localization | Cytoplasm. Nucleus. Note=Colocalizes with DLC1 at actin-rich regions in the cell periphery |
| Entrez Gene | 1915 |
|---|
Application
Western blot analysis of EEF1A1 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of EEF1A1 Antibody (C-term)(Cat#AP6592b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
EEF1A1 Antibody (C-term) (AP6592b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EEF1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
