Application
Western blot analysis of EEF1A1 Antibody (N-term) in Y79, T47D cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain with EEF1A1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
With 293T cell line lysate,resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EF1A1( abgent, Cat. AP6592a, 1:500, 4°C，overnight ) and horseradish peroxidase–conjugated second antibo dy(rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).| Product Name | EEF1A1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EEF1A, EF1A, LENG7 |
|---|---|
| Alternative Names | EEF1A1 EEF1A EF1A LENG7 Elongation factor 1-alpha 1 Elongation factor Tu Eukaryotic elongation factor 1 A-1 Leukocyte receptor cluster member 7 |
| Molecular Weight(MW) | 50141 Da |
| Function | This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production |
| Tissue Specificity | Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle |
| Cellular Localization | Cytoplasm. Nucleus. Note=Colocalizes with DLC1 at actin-rich regions in the cell periphery |
| Entrez Gene | 1915 |
|---|
Application
Western blot analysis of EEF1A1 Antibody (N-term) in Y79, T47D cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain with EEF1A1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
With 293T cell line lysate,resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EF1A1( abgent, Cat. AP6592a, 1:500, 4°C，overnight ) and horseradish peroxidase–conjugated second antibo dy(rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
