Application
A. Western blot analysis on 293 cell lysate using anti-eIF-4E RabMAb (Cat. #AJ1255a), dilution 1:500Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-eIF-4E RabMAb (Cat. #AJ1255a).| Product Name | eIF-4E Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y448 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1: 20000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIF4EL1, EIF4F |
|---|---|
| Alternative Names | EIF4E EIF4EL1 EIF4F Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit |
| Molecular Weight(MW) | 25097 Da |
| Function | Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap |
| Tissue Specificity | A synthetic peptide corresponding to residues in human eIF-4E, was used as immunogen. The antibody detects a band on western blot of approximately 28 kDa. |
| Cellular Localization | Cytoplasm, P-body. |
| Entrez Gene | 1977 |
|---|
Application
A. Western blot analysis on 293 cell lysate using anti-eIF-4E RabMAb (Cat. #AJ1255a), dilution 1:500
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-eIF-4E RabMAb (Cat. #AJ1255a).| Application Notes | WB:1: 20000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
