Application
Immunofluorescence of monoclonal antibody to ENO3 (AT1911a) on HeLa cell . [antibody concentration 10 ug/ml]Application
Western blot analysis of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi ( Cat # H00002027-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody (M01), clone 5D1 (Cat # AT1911a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Product Name | ENO3 Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MSEenolase 3 (beta, muscle) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5D1 |
| Host Species | Mouse |
| Tested Applications | IFWB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MSEenolase 3 (beta muscle) |
|---|---|
| Molecular Weight(MW) | 46987 Da |
| Tissue Specificity | ENO3 (NP_001967, 228 a.a. ~ 278 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Application
Immunofluorescence of monoclonal antibody to ENO3 (AT1911a) on HeLa cell . [antibody concentration 10 ug/ml]
Application
Western blot analysis of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi ( Cat # H00002027-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody (M01), clone 5D1 (Cat # AT1911a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
