Application
Figure 1: Western blot analysis using anti-EphA1 monoclonal antiobdy against A549(1) and Hela(2) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human ovary carcinoma(left) and breast carcinoma(right), showing cytoplasmic localization using anti-EphA1 antibody with DAB staining.| Product Name | EphA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5D2 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EPH, EPHT, EPHT1 |
|---|---|
| Alternative Names | EPH EPHT EPHT1 |
| Molecular Weight(MW) | 108127 Da |
| Function | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis |
| Tissue Specificity | Overexpressed in several carcinomas. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 2041 |
|---|---|
| Nucleotide Accession | NP_005223.4 |
Application
Figure 1: Western blot analysis using anti-EphA1 monoclonal antiobdy against A549(1) and Hela(2) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human ovary carcinoma(left) and breast carcinoma(right), showing cytoplasmic localization using anti-EphA1 antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of EphA1 expressed in E. Coli. |
