Application
Western blot analysis of anti-EPHA5 Monoclonal Antibody (Cat.#AM7610a) by EPHA5 recombinant protein (Fragment). EPHA5 (Fragment) protein (arrow) was detected using the purified Mab.(1:2000)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody (Cat.#AM7610a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EPHA5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 46CT61.6.4 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BSK, EHK1, HEK7, TYRO4 |
|---|---|
| Alternative Names | EPHA5 BSK EHK1 HEK7 TYRO4 Ephrin type-A receptor 5 Brain-specific kinase EPH homology kinase 1 EPH-like kinase 7 |
| Molecular Weight(MW) | 114803 Da |
| Function | Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5 Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino- hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity) |
| Tissue Specificity | Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord |
| Cellular Localization | Cell membrane (By similarity); Single-pass type I membrane protein (By similarity). Cell projection, axon (By similarity). Cell projection, dendrite |
| Entrez Gene | 2044 |
|---|
Application
Western blot analysis of anti-EPHA5 Monoclonal Antibody (Cat.#AM7610a) by EPHA5 recombinant protein (Fragment). EPHA5 (Fragment) protein (arrow) was detected using the purified Mab.(1:2000)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody (Cat.#AM7610a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
