Application
Figure 1: Western blot analysis using anti-EphA4 monoclonal antibody against truncated Trx-EphA4 recombinant protein(1) and truncated GST-EphA4(aa777-986) recombinant protein(2).| Product Name | EphA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SEK; HEK8; TYRO1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7D3D4 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HEK8 SEK TYRO1 |
|---|---|
| Alternative Names | SEK HEK8 TYRO1 |
| Molecular Weight(MW) | 109860 Da |
| Function | Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development Beside its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, axon (By similarity) Cell projection, dendrite (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity) Early endosome (By similarity). Note=Clustered upon activation and targeted to early endosome (By similarity) |
| Entrez Gene | 2043 |
|---|---|
| Nucleotide Accession | NP_004429.1 |
Application
Figure 1: Western blot analysis using anti-EphA4 monoclonal antibody against truncated Trx-EphA4 recombinant protein(1) and truncated GST-EphA4(aa777-986) recombinant protein(2).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of EphA4(aa777-986) expressed in E. Coli. |
