Application
Figure 1: Western blot analysis using anti-EphA7 monoclonal antibody against truncated GST-EphA7 recombinant protein(1) and truncated EphA7(aa25-556)-hIgGFc transfected CHOK1 cell lysate (2).| Product Name | EphA7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 6C8G7 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EHK3, HEK11 |
|---|---|
| Alternative Names | EHK3 HEK11 |
| Molecular Weight(MW) | 112097 Da |
| Function | Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7 |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Cell membrane (Probable); Single-pass type I membrane protein (Probable) |
| Entrez Gene | 2045 |
|---|---|
| Nucleotide Accession | NP_004431.1 |
Application
Figure 1: Western blot analysis using anti-EphA7 monoclonal antibody against truncated GST-EphA7 recombinant protein(1) and truncated EphA7(aa25-556)-hIgGFc transfected CHOK1 cell lysate (2).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of EphA7(aa27-210) expressed in E. Coli. |
