Application
Figure 1: Western blot analysis using anti-EphA8 monoclonal antibody against truncated Trx-EphA8 recombinant protein(1) and truncated MBP-EphA8(aa70-150) recombinant protein(2).| Product Name | EphA8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9A12D8 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EEK, HEK3, KIAA1459 |
|---|---|
| Alternative Names | EEK HEK3 KIAA1459 EPHA8 |
| Molecular Weight(MW) | 111003 Da |
| Function | Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The GPI-anchored ephrin-A EFNA2, EFNA3, and EFNA5 are able to activate EPHA8 through phosphorylation. With EFNA5 may regulate integrin-mediated cell adhesion and migration on fibronectin substrate but also neurite outgrowth. During development of the nervous system plays also a role in axon guidance. Downstream effectors of the EPHA8 signaling pathway include FYN which promotes cell adhesion upon activation by EPHA8 and the MAP kinases in the stimulation of neurite outgrowth (By similarity) |
| Tissue Specificity | Purified recombinant fragment of EphA8(aa70-150) expressed in E. Coli. |
| Cellular Localization | Cell membrane (By similarity); Single-pass type I membrane protein (By similarity). Cell projection (By similarity). Early endosome membrane (By similarity) Note=Undergoes clathrin-mediated endocytosis upon EFNA5-binding and is targeted to early endosomes (By similarity) |
| Entrez Gene | 2046 |
|---|---|
| Nucleotide Accession | NP_065387.1 |
Application
Figure 1: Western blot analysis using anti-EphA8 monoclonal antibody against truncated Trx-EphA8 recombinant protein(1) and truncated MBP-EphA8(aa70-150) recombinant protein(2).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of EphA8(aa70-150) expressed in E. Coli. |
