Application
Western blot analysis of anti-EphB2 C-term Pab in NCI-H460 cell lysate. EphB2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EphB2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of EphB2 Antibody (C-term)(Cat#AP7623b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | EphB2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5 |
|---|---|
| Alternative Names | EPHB2 DRT EPHT3 EPTH3 ERK HEK5 TYRO5 Ephrin type-B receptor 2 Developmentally-regulated Eph-related tyrosine kinase ELK-related tyrosine kinase EPH tyrosine kinase 3 EPH-like kinase 5 Renal carcinoma antigen NY-REN-47 Tyrosine-protein kinase T |
| Molecular Weight(MW) | 117493 Da |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. Beside axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor |
| Tissue Specificity | Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cell projection, axon (By similarity). Cell projection, dendrite (By similarity) |
| Entrez Gene | 2048 |
|---|
Application
Western blot analysis of anti-EphB2 C-term Pab in NCI-H460 cell lysate. EphB2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EphB2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of EphB2 Antibody (C-term)(Cat#AP7623b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
