Application
Western blot analysis of ETHE1 Antibody (C-term) in mouse liver tissue lysates (35ug/lane).ETHE1 (arrow) was detected using the purified Pab.Application
(LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ETHE1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of 293 cells using ETHE1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ETHE1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ETHE1; HSCO; Protein ETHE1, mitochondrial; Ethylmalonic encephalopathy protein 1; Hepatoma subtracted clone one protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSCO |
|---|---|
| Alternative Names | ETHE1 HSCO Protein ETHE1 mitochondrial Ethylmalonic encephalopathy protein 1 Hepatoma subtracted clone one protein |
| Molecular Weight(MW) | 27873 Da |
| Function | Probably plays an important role in metabolic homeostasis in mitochondria. May function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. Suppresses p53- induced apoptosis by preventing nuclear localization of RELA |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Cytoplasm. Nucleus. Mitochondrion matrix. Note=According to PubMed:12398897, it is cytoplasmic and nuclear According to PubMed:14732903, it is found in the mitochondrial matrix |
| Entrez Gene | 23474 |
|---|
Application
Western blot analysis of ETHE1 Antibody (C-term) in mouse liver tissue lysates (35ug/lane).ETHE1 (arrow) was detected using the purified Pab.
Application
(LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ETHE1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of 293 cells using ETHE1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
