Application
Western blot analysis of ETHE1 antibody (Center) in mouse kidney tissue lysates (35ug/lane). ETHE1 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of 293 cells using ETHE1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
ETHE1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ETHE1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of ETHE1 (arrow) using rabbit polyclonal ETHE1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ETHE1 gene.| Product Name | ETHE1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIHC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSCO |
|---|---|
| Alternative Names | ETHE1 HSCO Protein ETHE1 mitochondrial Ethylmalonic encephalopathy protein 1 Hepatoma subtracted clone one protein |
| Molecular Weight(MW) | 27873 Da |
| Function | Probably plays an important role in metabolic homeostasis in mitochondria. May function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. Suppresses p53- induced apoptosis by preventing nuclear localization of RELA |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Cytoplasm. Nucleus. Mitochondrion matrix. Note=According to PubMed:12398897, it is cytoplasmic and nuclear According to PubMed:14732903, it is found in the mitochondrial matrix |
| Entrez Gene | 23474 |
|---|
Application
Western blot analysis of ETHE1 antibody (Center) in mouse kidney tissue lysates (35ug/lane). ETHE1 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of 293 cells using ETHE1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
ETHE1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ETHE1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of ETHE1 (arrow) using rabbit polyclonal ETHE1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ETHE1 gene.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
