Application
A. Western blot analysis on Jurkat cell lysate using anti-Ezh 1/2 RabMAb (Cat. #AJ1278a), dilution 1:500.Application
B. Immunofluorescent staining of HeLa cells using anti-ezh RabMAb (Cat. #AJ1278a).| Product Name | Ezh Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EZH2, KMT6, Histone-lysine N-methyltransferase EZH2, Enhancer of zeste homolog 2;ENX-1;Lysine N-methyltransferase 6 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1408Y |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:1000~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT6 |
|---|---|
| Alternative Names | EZH2 KMT6 Histone-lysine N-methyltransferase EZH2 Enhancer of zeste homolog 2 ENX-1 Lysine N-methyltransferase 6 |
| Molecular Weight(MW) | 85363 Da |
| Function | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 |
| Tissue Specificity | Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2146 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Ezh 1/2 RabMAb (Cat. #AJ1278a), dilution 1:500.
Application
B. Immunofluorescent staining of HeLa cells using anti-ezh RabMAb (Cat. #AJ1278a).| Application Notes | WB:1:1000~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
