Application
EZH2 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the EZH2 antibody detected the EZH2 protein (arrow).Application
EZH2 Antibody (N-term) western blot analysis in MDA-MB468 cell line lysates (35ug/lane).This demonstrates the EZH2 antibody detected the EZH2 protein (arrow).| Product Name | EZH2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EZH2; KMT6; Histone-lysine N-methyltransferase EZH2; ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT6 |
|---|---|
| Alternative Names | EZH2 KMT6 Histone-lysine N-methyltransferase EZH2 ENX-1 Enhancer of zeste homolog 2 Lysine N-methyltransferase 6 |
| Molecular Weight(MW) | 85363 Da |
| Function | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 |
| Tissue Specificity | Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2146 |
|---|---|
| Nucleotide Accession | NP_004447.2 NP_694543.1 |
Application
EZH2 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the EZH2 antibody detected the EZH2 protein (arrow).
Application
EZH2 Antibody (N-term) western blot analysis in MDA-MB468 cell line lysates (35ug/lane).This demonstrates the EZH2 antibody detected the EZH2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
