Application
Western blot analysis of EZH2 antibody (Cat. #AM1836a) in T47D cell line lysates (35ug/lane).EZH2 (arrow) was detected using the purified Mab.Application
EZH2 Monoclonal Antibody (Cat. #AM1836a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EZH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of EZH2 Antibody (Cat#AM1836a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | EZH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EZH2; KMT6; Histone-lysine N-methyltransferase EZH2; ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 144CT2.1.1.5 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT6 |
|---|---|
| Alternative Names | EZH2 KMT6 Histone-lysine N-methyltransferase EZH2 ENX-1 Enhancer of zeste homolog 2 Lysine N-methyltransferase 6 |
| Molecular Weight(MW) | 85363 Da |
| Function | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 |
| Tissue Specificity | Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2146 |
|---|
Application
Western blot analysis of EZH2 antibody (Cat. #AM1836a) in T47D cell line lysates (35ug/lane).EZH2 (arrow) was detected using the purified Mab.
Application
EZH2 Monoclonal Antibody (Cat. #AM1836a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EZH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of EZH2 Antibody (Cat#AM1836a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
