Application
Western blot analysis of FLI1 over-expressed 293 cell line, cotransfected with FLI1 Validated Chimera RNAi ( Cat # H00002313-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FLI1 monoclonal antibody (M05), clone 1F6 (Cat # AT2060a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Application
Western Blot analysis of FLI1 expression in transfected 293T cell line by FLI1 monoclonal antibody (M05), clone 1F6.Lane 1: FLI1 transfected lysate(50.982 KDa).Lane 2: Non-transfected lysate.| Product Name | FLI1 Antibody (monoclonal) (M05) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EWSR2, SIC1Friend leukemia virus integration 1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 1F6 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EWSR2 SIC1Friend leukemia virus integration 1 |
|---|---|
| Molecular Weight(MW) | 50982 Da |
| Tissue Specificity | FLI1 (AAH01670, 1 a.a. ~ 453 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Entrez Gene | 2313 |
|---|
Application
Western blot analysis of FLI1 over-expressed 293 cell line, cotransfected with FLI1 Validated Chimera RNAi ( Cat # H00002313-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FLI1 monoclonal antibody (M05), clone 1F6 (Cat # AT2060a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Application
Western Blot analysis of FLI1 expression in transfected 293T cell line by FLI1 monoclonal antibody (M05), clone 1F6.Lane 1: FLI1 transfected lysate(50.982 KDa).Lane 2: Non-transfected lysate.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
