Application
Figure 1: Western blot analysis using FMR1 mouse mAb against Jurkat (1) and K562 (2) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain tissues, showing cytoplasmic localization with DAB staining using FMR1 mouse mAb.Application
Figure 3: Immunofluorescence analysis of HIN-3T3 cells using FMR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Product Name | FMR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POF; FMRP; POF1; FRAXA |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4G9 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | POF FMRP POF1 FRAXA |
|---|---|
| Molecular Weight(MW) | 71174 Da |
| Function | Translation repressor. Component of the CYFIP1-EIF4E- FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA- binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C) |
| Tissue Specificity | Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells |
| Cellular Localization | Cytoplasm. Nucleus, nucleolus. |
| Entrez Gene | 2332 |
|---|---|
| Nucleotide Accession | NP_002015.1 |
Application
Figure 1: Western blot analysis using FMR1 mouse mAb against Jurkat (1) and K562 (2) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain tissues, showing cytoplasmic localization with DAB staining using FMR1 mouse mAb.
Application
Figure 3: Immunofluorescence analysis of HIN-3T3 cells using FMR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human FMR1 expressed in E. Coli. |
