Application
Western blot analysis of FRK (arrow) using rabbit polyclonal FRK Antibody (V28) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FRK gene.Application
FRK Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FRK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
FRK Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | FRK Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FRK; PTK5; RAK; Tyrosine-protein kinase FRK; FYN-related kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTK5 RAK |
|---|---|
| Alternative Names | FRK PTK5 RAK Tyrosine-protein kinase FRK FYN-related kinase Nuclear tyrosine protein kinase RAK Protein-tyrosine kinase 5 |
| Molecular Weight(MW) | 58254 Da |
| Function | Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor |
| Tissue Specificity | Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon |
| Cellular Localization | Cytoplasm. Nucleus. Note=Predominantly found in the nucleus, with a small fraction found in the cell periphery |
| Entrez Gene | 2444 |
|---|
Application
Western blot analysis of FRK (arrow) using rabbit polyclonal FRK Antibody (V28) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FRK gene.
Application
FRK Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FRK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
FRK Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
