Application
G3BP1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the G3BP1 antibody detected the G3BP1 protein (arrow).Application
G3BP1 Antibody (C-term) (AP13785b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of G3BP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | G3BP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G3BP |
|---|---|
| Alternative Names | G3BP1 G3BP Ras GTPase-activating protein-binding protein 1 ATP-dependent DNA helicase VIII GAP SH3 domain-binding protein 1 |
| Molecular Weight(MW) | 52164 Da |
| Function | May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium- dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Cytoplasm, cytosol. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS |
| Entrez Gene | 10146 |
|---|---|
| Nucleotide Accession | NP_938405.1 NP_005745.1 |
Application
G3BP1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the G3BP1 antibody detected the G3BP1 protein (arrow).
Application
G3BP1 Antibody (C-term) (AP13785b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of G3BP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
