Application
A. Western blot analysis on (A) HeLa, (B) SW480, (C) A431 and (D) MCF7 cell lysates using anti-Galectin-3 RabMAb (Cat. #AJ1312b), dilution 1:5000.Application
B.Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using anti-Galectin-3 RabMAb (Cat. #AJ1312b).Application
C. Immunofluorescent staining of HeLa cells using anti-Galectin-3 RabMAb (Cat. #AJ1312b).| Product Name | Galectin-3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2774 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAC2 |
|---|---|
| Alternative Names | LGALS3 MAC2 Galectin-3 Laminin-binding protein Lectin L-29 L-31 Galactoside-binding protein |
| Molecular Weight(MW) | 26152 Da |
| Function | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells |
| Tissue Specificity | A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages |
| Cellular Localization | Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface |
| Entrez Gene | 3958 |
|---|
Application
A. Western blot analysis on (A) HeLa, (B) SW480, (C) A431 and (D) MCF7 cell lysates using anti-Galectin-3 RabMAb (Cat. #AJ1312b), dilution 1:5000.
Application
B.Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using anti-Galectin-3 RabMAb (Cat. #AJ1312b).
Application
C. Immunofluorescent staining of HeLa cells using anti-Galectin-3 RabMAb (Cat. #AJ1312b).| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
