Application
LGALS3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the LGALS3 antibody detected the LGALS3 protein (arrow).Application
LGALS3 Antibody (C-term) (AP13725b)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LGALS3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | LGALS3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LGALS3; MAC2; Galectin-3; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAC2 |
|---|---|
| Alternative Names | LGALS3 MAC2 Galectin-3 35 kDa lectin Carbohydrate-binding protein 35 Galactose-specific lectin 3 Galactoside-binding protein IgE-binding protein L-31 Laminin-binding protein Lectin L-29 Mac-2 antigen |
| Molecular Weight(MW) | 26152 Da |
| Function | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells |
| Tissue Specificity | A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages |
| Cellular Localization | Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface |
| Entrez Gene | 3958 |
|---|---|
| Nucleotide Accession | NP_002297.2 |
Application
LGALS3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the LGALS3 antibody detected the LGALS3 protein (arrow).
Application
LGALS3 Antibody (C-term) (AP13725b)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LGALS3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
