Application
GFAP Antibody (Cat. #AM1870b) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the GFAP antibody detected the GFAP protein (arrow).Application
GFAP Monoclonal antibody (Cat. #AM1870b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFAP Monoclonal antibody( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of GFAP Antibody (Cat#AM1870b) with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | GFAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 183CT3.1.5 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GFAP Glial fibrillary acidic protein |
|---|---|
| Molecular Weight(MW) | 49880 Da |
| Function | GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells |
| Tissue Specificity | Expressed in cells lacking fibronectin. |
| Cellular Localization | Cytoplasm. Note=Associated with intermediate filaments |
| Entrez Gene | 2670 |
|---|---|
| Nucleotide Accession | NP_001124491.1 |
Application
GFAP Antibody (Cat. #AM1870b) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the GFAP antibody detected the GFAP protein (arrow).
Application
GFAP Monoclonal antibody (Cat. #AM1870b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFAP Monoclonal antibody( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of GFAP Antibody (Cat#AM1870b) with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
