Application
GFER Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GFER antibody detected the GFER protein (arrow).Application
GFER Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | GFER Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ALR, HERV1, HPO |
|---|---|
| Alternative Names | GFER ALR HERV1 HPO FAD-linked sulfhydryl oxidase ALR Augmenter of liver regeneration Hepatopoietin |
| Molecular Weight(MW) | 23449 Da |
| Function | Isoform 1: FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re- oxidized by donating electrons to cytochrome c or molecular oxygen |
| Tissue Specificity | Ubiquitously expressed. Highest expression in the testis and liver and low expression in the muscle |
| Cellular Localization | Isoform 1: Mitochondrion intermembrane space |
| Entrez Gene | 2671 |
|---|---|
| Nucleotide Accession | NP_005253.3 |
Application
GFER Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GFER antibody detected the GFER protein (arrow).
Application
GFER Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
