Application
A. Western blot analysis on (A) SKBR-3 cell lysate and (B) 3T3 cell lysate using anti-GOS-28 RabMAb (Cat. #AJ1329a), dilution 1:500.Application
B. Immmunohistochemical analysis of paraffin-embedded human stomach carcinoma using anti-GOS-28 RabMAb (Cat. #AJ1329a).Application
C. Immunofluorescent staining of HeLa cells using anti-GOS-28 RabMAb (Cat. #AJ1329a).| Product Name | GOS-28 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1768Y |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GS28 |
|---|---|
| Molecular Weight(MW) | 28613 Da |
| Function | Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide- sensitive factor) attachment protein receptor. May play a protective role against hydrogen peroxide induced cytotoxicity under glutathione depleted conditions in neuronal cells by regulating the intracellular ROS levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13 and MAPK14). Participates in docking and fusion stage of ER to cis-Golgi transport. Plays an important physiological role in VLDL-transport vesicle-Golgi fusion and thus in VLDL delivery to the hepatic cis-Golgi |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-terminus of human GOS-28 was used as an immunogen. |
| Cellular Localization | Golgi apparatus membrane; Single-pass type IV membrane protein. Note=Enriched on vesicular components at the terminal rims of the Golgi. Found in Golgi microtubules at low temperature (15 degrees Celsius) |
| Entrez Gene | 9527 |
|---|
Application
A. Western blot analysis on (A) SKBR-3 cell lysate and (B) 3T3 cell lysate using anti-GOS-28 RabMAb (Cat. #AJ1329a), dilution 1:500.
Application
B. Immmunohistochemical analysis of paraffin-embedded human stomach carcinoma using anti-GOS-28 RabMAb (Cat. #AJ1329a).
Application
C. Immunofluorescent staining of HeLa cells using anti-GOS-28 RabMAb (Cat. #AJ1329a).| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
