Application
Figure 1: Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.Application
Figure 2:Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Product Name | GOT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KAT4; KATIV; mitAAT |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3000000000 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | KAT4 KATIV mitAAT |
|---|---|
| Molecular Weight(MW) | 47518 Da |
| Function | Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA) Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids |
| Tissue Specificity | Purified recombinant fragment of human GOT2 expressed in E. Coli. |
| Cellular Localization | Mitochondrion matrix. Cell membrane. Note=Exposure to alcohol promotes translocation to the cell membrane |
| Entrez Gene | 2806 |
|---|---|
| Nucleotide Accession | NP_002071.2 |
Application
Figure 1: Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
Application
Figure 2:Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human GOT2 expressed in E. Coli. |
