Application
Figure 1: Western blot analysis using anti-GSK3 alpha monoclonal antiobdy against truncated GSK3 alpha recombinant protein.Application
Figure 2: Immunofluorescence analysis of Hela cells using anti-GSK3 alpha antibody showing cytoplasmic localization.| Product Name | GSK3 alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686D0638; GSK3A |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 9D5G1 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp686D0638 GSK3A |
|---|---|
| Molecular Weight(MW) | 50981 Da |
| Function | Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1 Requires primed phosphorylation of the majority of its substrates Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth |
| Tissue Specificity | Purified recombinant fragment of GSK3 alpha expressed in E. Coli. |
| Entrez Gene | 2931 |
|---|---|
| Nucleotide Accession | NP_063937.2 |
Application
Figure 1: Western blot analysis using anti-GSK3 alpha monoclonal antiobdy against truncated GSK3 alpha recombinant protein.
Application
Figure 2: Immunofluorescence analysis of Hela cells using anti-GSK3 alpha antibody showing cytoplasmic localization.| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of GSK3 alpha expressed in E. Coli. |
