Application
H2AFX Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the H2AFX antibody detected the H2AFX protein (arrow).Application
H2AFX Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of H2AFX Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | H2AFX Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2AFX; H2AX; Histone H2A.x |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H2AX |
|---|---|
| Alternative Names | H2AFX H2AX Histone H2A.x |
| Molecular Weight(MW) | 15145 Da |
| Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation |
| Tissue Specificity | This H2AFX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-140 amino acids from the C-terminal region of human H2AFX. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 3014 |
|---|---|
| Nucleotide Accession | NP_002096.1 |
Application
H2AFX Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the H2AFX antibody detected the H2AFX protein (arrow).
Application
H2AFX Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of H2AFX Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
