Application
Western blot analysis of H2AFX Antibody (C-term) in CEM, 293 cell line lysates (35ug/lane). H2AFX (arrow) was detected using the purified Pab.Application
H2AFX Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the H2AFX Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
H2AFX Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | H2AFX Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2AFX; H2AX; Histone H2A.x |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H2AX |
|---|---|
| Alternative Names | H2AFX H2AX Histone H2A.x |
| Molecular Weight(MW) | 15145 Da |
| Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation |
| Tissue Specificity | This H2AFX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114~143 amino acids from the C-terminal region of human FCGR2A. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 3014 |
|---|
Application
Western blot analysis of H2AFX Antibody (C-term) in CEM, 293 cell line lysates (35ug/lane). H2AFX (arrow) was detected using the purified Pab.
Application
H2AFX Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the H2AFX Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
H2AFX Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
