Application
Western blot analysis of HAND2 over-expressed 293 cell line, cotransfected with HAND2 Validated Chimera RNAi ( Cat # H00009464-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HAND2 monoclonal antibody (M06), clone 3D5 (Cat # AT2315a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Application
Western Blot analysis of HAND2 expression in transfected 293T cell line by HAND2 monoclonal antibody (M06), clone 3D5.Lane 1: HAND2 transfected lysate(21.6 KDa).Lane 2: Non-transfected lysate.| Product Name | HAND2 Antibody (monoclonal) (M06) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DHAND2, Hed, MGC125303, MGC125304, Thing2, dHandheart and neural crest derivatives expressed 2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3D5 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | DHAND2 Hed MGC125303 MGC125304 Thing2 dHandheart and neural crest derivatives expressed 2 |
|---|---|
| Molecular Weight(MW) | 23666 Da |
| Tissue Specificity | HAND2 (NP_068808, 135 a.a. ~ 217 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Application
Western blot analysis of HAND2 over-expressed 293 cell line, cotransfected with HAND2 Validated Chimera RNAi ( Cat # H00009464-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HAND2 monoclonal antibody (M06), clone 3D5 (Cat # AT2315a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Application
Western Blot analysis of HAND2 expression in transfected 293T cell line by HAND2 monoclonal antibody (M06), clone 3D5.Lane 1: HAND2 transfected lysate(21.6 KDa).Lane 2: Non-transfected lysate.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
