Application
Figure 1: Western blot analysis using anti-HCK monoclonal antiobdy against truncated HCK recombinant protein(1) and full-length HCK-GFP transfected CHO-K1 cell lysate(2).Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human colon cancer(left) and ancreas cancer(right), showing cytoplasmic localization using anti-HCK antibody with DAB staining.| Product Name | HCK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JTK9 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3D12E10 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | JTK9 |
|---|---|
| Molecular Weight(MW) | 59600 Da |
| Function | Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS |
| Tissue Specificity | Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes Detected in tonsil |
| Cellular Localization | Isoform 1: Lysosome. Membrane; Lipid-anchor. Cell projection, podosome membrane; Lipid-anchor. Cytoplasm, cytosol. Note=Associated with specialized secretory lysosomes called azurophil granules. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol |
| Entrez Gene | 3055 |
|---|---|
| Nucleotide Accession | NP_002101.2 |
Application
Figure 1: Western blot analysis using anti-HCK monoclonal antiobdy against truncated HCK recombinant protein(1) and full-length HCK-GFP transfected CHO-K1 cell lysate(2).
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human colon cancer(left) and ancreas cancer(right), showing cytoplasmic localization using anti-HCK antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of HCK expressed in E. Coli. |
