Application
Western blot analysis of HFE antibody (Center) (Cat.# AP6544c) in Jurkat cell line lysates (35ug/lane). HFE (arrow) was detected using the purified Pab.Application
HFE Antibody (Center) (Cat.# AP6544c) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HFE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | HFE Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HFE; HLAH; Hereditary hemochromatosis protein; HLA-H |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HLAH |
|---|---|
| Alternative Names | HFE HLAH Hereditary hemochromatosis protein HLA-H |
| Molecular Weight(MW) | 40108 Da |
| Function | Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin |
| Tissue Specificity | Expressed in all tissues tested except brain. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3077 |
|---|
Application
Western blot analysis of HFE antibody (Center) (Cat.# AP6544c) in Jurkat cell line lysates (35ug/lane). HFE (arrow) was detected using the purified Pab.
Application
HFE Antibody (Center) (Cat.# AP6544c) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HFE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
