Application
Western blot analysis of HFE antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). HFE (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of NCI-H292 cells using HFE Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of HFE Antibody (N-term)(Cat#AP6544a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | HFE Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HLAH |
|---|---|
| Alternative Names | HFE HLAH Hereditary hemochromatosis protein HLA-H |
| Molecular Weight(MW) | 40108 Da |
| Function | Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin |
| Tissue Specificity | Expressed in all tissues tested except brain. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3077 |
|---|
Application
Western blot analysis of HFE antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). HFE (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of NCI-H292 cells using HFE Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of HFE Antibody (N-term)(Cat#AP6544a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
