Application
HIST1H2BJ antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2BJ antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
HIST1H2BJ Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
HIST1H2BJ Antibody (Center) western blot analysis in CEM,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST1H2BJ antibody detected the HIST1H2BJ protein (arrow).| Product Name | HIST1H2BJ Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCFCWB |
| IHC:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H2BFR |
|---|---|
| Alternative Names | HIST1H2BJ H2BFR Histone H2B type 1-J Histone H2B.1 Histone H2B.r |
| Molecular Weight(MW) | 13904 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | This HIST1H2BJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the Central region of human HIST1H2BJ. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8970 |
|---|---|
| Nucleotide Accession | NP_066402.2 |
Application
HIST1H2BJ antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2BJ antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
HIST1H2BJ Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
HIST1H2BJ Antibody (Center) western blot analysis in CEM,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST1H2BJ antibody detected the HIST1H2BJ protein (arrow).| Application Notes | IHC:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
