Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-H2A.x (pS139) RabMAb (Cat. #AJ1351a). Cells were either (A) untreated (B) treated with etoposideApplication
B. Immunohistochemical analysis of paraffin-embedded human kidney transitional cell carcinoma using anti-Histone H2A.x RabMAb (Cat. #AJ1351a).| Product Name | Histone H2A.x Antibody Phospho (pS139) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S139 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP854(2)Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H2AX |
|---|---|
| Alternative Names | H2AFX H2AX Histone H2A.x |
| Molecular Weight(MW) | 15145 Da |
| Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding serine 139 of human H2A.x protein. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 3014 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-H2A.x (pS139) RabMAb (Cat. #AJ1351a). Cells were either (A) untreated (B) treated with etoposide
Application
B. Immunohistochemical analysis of paraffin-embedded human kidney transitional cell carcinoma using anti-Histone H2A.x RabMAb (Cat. #AJ1351a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
