Application
Western blot analysis of anti-HH3R Pab (Rabbit ID 1071) in Jurkat cell line lysate (35ug/lane). HH3R(arrow) was detected using the purified Pab (1:60 dilution). This western blot identifies isoform two of HRH3. The accession number of HRH3 is CAC39434; Q9Y5N1.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HRH3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | HRH3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HRH3; GPCR97; Histamine H3 receptor; G-protein coupled receptor 97 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GPCR97 |
|---|---|
| Alternative Names | HRH3 GPCR97 Histamine H3 receptor G-protein coupled receptor 97 |
| Molecular Weight(MW) | 48671 Da |
| Function | The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 neither modified adenylate cyclase activity nor induced intracellular calcium mobilization |
| Tissue Specificity | Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| Entrez Gene | 11255 |
|---|
Application
Western blot analysis of anti-HH3R Pab (Rabbit ID 1071) in Jurkat cell line lysate (35ug/lane). HH3R(arrow) was detected using the purified Pab (1:60 dilution). This western blot identifies isoform two of HRH3. The accession number of HRH3 is CAC39434; Q9Y5N1.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HRH3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
