Application
Western blot analysis of anti-HSF2 Sumoylation Site Antibody in HepG2 cell line lysates (35ug/lane). HSF2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with HSF2 Sumoylation Site antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | HSF2 Sumoylation Site Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSF2; HSTF2; Heat shock factor protein 2; Heat shock transcription factor 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSTF2 |
|---|---|
| Alternative Names | HSF2 HSTF2 Heat shock factor protein 2 Heat shock transcription factor 2 |
| Molecular Weight(MW) | 60348 Da |
| Function | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked |
| Tissue Specificity | This HSF2 Sumoylation Site antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide VHIDSGIVKQERDGP containing a predicted sumoylation site from the N-terminal region of human HSF2. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation |
| Entrez Gene | 3298 |
|---|
Application
Western blot analysis of anti-HSF2 Sumoylation Site Antibody in HepG2 cell line lysates (35ug/lane). HSF2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with HSF2 Sumoylation Site antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
