Application
Western blot analysis of ID2 over-expressed 293 cell line, cotransfected with ID2 Validated Chimera RNAi ( Cat # H00003398-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ID2 monoclonal antibody (M01), clone 3C3 (Cat # AT2475a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Application
Western Blot analysis of ID2 expression in transfected 293T cell line by ID2 monoclonal antibody (M01), clone 3C3.Lane 1: ID2 transfected lysate(14.9 KDa).Lane 2: Non-transfected lysate.| Product Name | ID2 Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GIG8, ID2A, ID2H, MGC26389inhibitor of DNA binding 2, dominant negative helix-loop-helix protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3C3 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GIG8 ID2A ID2H MGC26389inhibitor of DNA binding 2 dominant negative helix-loop-helix protein |
|---|---|
| Molecular Weight(MW) | 14917 Da |
| Tissue Specificity | ID2 (AAH30639, 1 a.a. ~ 135 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Entrez Gene | 3398 |
|---|
Application
Western blot analysis of ID2 over-expressed 293 cell line, cotransfected with ID2 Validated Chimera RNAi ( Cat # H00003398-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ID2 monoclonal antibody (M01), clone 3C3 (Cat # AT2475a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Application
Western Blot analysis of ID2 expression in transfected 293T cell line by ID2 monoclonal antibody (M01), clone 3C3.Lane 1: ID2 transfected lysate(14.9 KDa).Lane 2: Non-transfected lysate.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
