Application
A. Western blot analysis on K562 cell lysate using anti-IFN-alpha R1 RabMAb (Cat. #AJ1388a), dilution 1:20,000.Application
B. Flow cytometric analysis of permeabilized K562 cells using anti-IFN-alpha R1 RabMAb (red) (Cat. #AJ1388a) or a rabbit IgG (negative) (green).| Product Name | IFN-alpha R1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IFNAR1, IFNAR, Interferon-alpha/beta receptor alpha chain |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP899Y |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:1000~10000 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IFNAR |
|---|---|
| Alternative Names | IFNAR1 IFNAR Interferon-alpha/beta receptor alpha chain |
| Molecular Weight(MW) | 63525 Da |
| Function | Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta- subunits themselves |
| Tissue Specificity | IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R |
| Cellular Localization | Isoform 1: Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3454 |
|---|
Application
A. Western blot analysis on K562 cell lysate using anti-IFN-alpha R1 RabMAb (Cat. #AJ1388a), dilution 1:20,000.
Application
B. Flow cytometric analysis of permeabilized K562 cells using anti-IFN-alpha R1 RabMAb (red) (Cat. #AJ1388a) or a rabbit IgG (negative) (green).| Application Notes | WB:1:1000~10000 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
