Application
Western blot analysis of IFNAR1 Antibody (Center) in K562 cell line lysates (35ug/lane). IFNAR1 (arrow) was detected using the purified Pab.(8ug/ml)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with IFNAR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using IFNAR1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IFNAR1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IFNAR1; IFNAR; Interferon alpha/beta receptor 1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; Type I interferon receptor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IFNAR |
|---|---|
| Alternative Names | IFNAR1 IFNAR Interferon alpha/beta receptor 1 Cytokine receptor class-II member 1 Cytokine receptor family 2 member 1 Type I interferon receptor 1 |
| Molecular Weight(MW) | 63525 Da |
| Function | Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta- subunits themselves |
| Tissue Specificity | IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R |
| Cellular Localization | Isoform 1: Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3454 |
|---|
Application
Western blot analysis of IFNAR1 Antibody (Center) in K562 cell line lysates (35ug/lane). IFNAR1 (arrow) was detected using the purified Pab.(8ug/ml)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with IFNAR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using IFNAR1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
