Application
A. Western blot analysis of Hela cell lysate using anti-IkappaB-alpha RabMAb (Cat. #AJ1389a), 1:10,000 dilution.Application
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-IkappaB-alpha RabMAb (Cat. #AJ1389a).Application
C. Flow cytometric analysis of permeabilized HeLa cells, untreated (green), TNF-a-treated (red), using anti-IkappaB-alpha RabMAb (Cat. #AJ1389a).| Product Name | IkappaB alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFKBIA, IKBA, MAD3, NFKBI, NF-kappa-B inhibitor alpha, Major histocompatibility complex enhancer-binding protein MAD3 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E130 |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IKBA MAD3 NFKBI |
|---|---|
| Alternative Names | NFKBIA IKBA MAD3 NFKBI NF-kappa-B inhibitor alpha Major histocompatibility complex enhancer-binding protein MAD3 |
| Molecular Weight(MW) | 35609 Da |
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription |
| Tissue Specificity | A synthetic peptide corresponding to human IkappaB-alpha was used as immunogen. Predicted to cross-react with pig, bovine, mouse and rat, based on sequence homology. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export (By similarity) |
| Entrez Gene | 4792 |
|---|
Application
A. Western blot analysis of Hela cell lysate using anti-IkappaB-alpha RabMAb (Cat. #AJ1389a), 1:10,000 dilution.
Application
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-IkappaB-alpha RabMAb (Cat. #AJ1389a).
Application
C. Flow cytometric analysis of permeabilized HeLa cells, untreated (green), TNF-a-treated (red), using anti-IkappaB-alpha RabMAb (Cat. #AJ1389a).| Application Notes | WB:1:10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
