Application
Western blot analysis on Hela cell lysates using anti-Phospho-IkappaB-alpha (pS32) RabMAb (Cat. #AJ1389d). Cells were either (A) untreated (B) treated with Calyculin A and TNF-a| Product Name | IkappaB-alpha Antibody Phospho (S32) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S32 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3148 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100010000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IKBA MAD3 NFKBI |
|---|---|
| Molecular Weight(MW) | 35609 Da |
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding Serine 32 of human IkappaB-alpha was used as an immunogen. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export (By similarity) |
| Entrez Gene | 4792 |
|---|
Application
Western blot analysis on Hela cell lysates using anti-Phospho-IkappaB-alpha (pS32) RabMAb (Cat. #AJ1389d). Cells were either (A) untreated (B) treated with Calyculin A and TNF-a| Application Notes | WB:1:100010000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
