Application
The anti-IKB-alpha N-term Pab is used in Western blot to detect IKB-alpha in Jurkat cell lysate.| Product Name | IKB alpha (IKBA) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; Major histocompatibility complex enhancer-binding protein MAD3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IKBA MAD3 NFKBI |
|---|---|
| Alternative Names | NFKBIA IKBA MAD3 NFKBI NF-kappa-B inhibitor alpha I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 |
| Molecular Weight(MW) | 35609 Da |
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription |
| Tissue Specificity | This IKBA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human IKB-alpha around the sumoylation site. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export (By similarity) |
Application
The anti-IKB-alpha N-term Pab is used in Western blot to detect IKB-alpha in Jurkat cell lysate.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
